用Python3+PyQt5编写程序绑定信号出错

用Python3+PyQt5编写程序时，给按钮绑定自己写的函数时，函数中的一部分代码报错详细请参考下面代码：

import sys
import threading
from PyQt5.QtWidgets import QMainWindow, QApplication, QPushButton, QHBoxLayout, QWidget
import conn_com
from configparser import ConfigParser

class MainWind(QMainWindow):
    def __init__(self,parent=None):
        super(MainWind,self).__init__(parent)
        self.setWindowTitle("Test")
        self.resize(714, 443)
        self.button1=QPushButton("Test")
        self.button1.clicked.connect(self.on_Click)
        layout = QHBoxLayout()
        layout.addWidget(self.button1)
        mainFrame = QWidget()
        mainFrame.setLayout(layout)
        self.setCentralWidget(mainFrame)

    def con_port(self):
        conn_com.Communication.__name__("/dev/ttys0", 115200, timeout=0.5)
        conn_com.Communication.Open_Port()
        conn_com.Communication.Print_Port()

    def Open_led(self):
        conf = ConfigParser()
        conf.read('settings/port_date.ini')
        opd = conf['date']['openLED']
        print(opd)
        return opd
        # conn_com.Communication.Send_data(opd)

    def on_Click(self,conf):
        self.sender()
        if __name__ == '__main__':
            conf
        print("sss")
        t3 = threading.Thread(target=self.Open_led, args=('t3',))
        t3.start()
        MainWind.instance()

if __name__=='__main__':
    app=QApplication(sys.argv)
    main=MainWind()
    main.show()
    sys.exit(app.exec_())

报错结果如下：

E:\Python_test\AutoTest_Tools\Scripts\python.exe "D:\Program Files\JetBrains\PyCharm Community Edition 2020.3.2\plugins\python-ce\helpers\pydev\pydevd.py" --multiproc --qt-support=auto --client 127.0.0.1 --port 59444 --file E:/AutoTest_Tools/qt/QWind.py
Connected to pydev debugger (build 211.7628.24)
sss
python-BaseException
python-BaseException
Traceback (most recent call last):
  File "D:\Python\lib\threading.py", line 865, in run
Traceback (most recent call last):
  File "E:/AutoTest_Tools/qt/QWind.py", line 40, in on_Click
    MainWind.instance()
AttributeError: type object 'MainWind' has no attribute 'instance'
    self._target(*self._args, **self._kwargs)
TypeError: Open_led() takes 1 positional argument but 2 were given

我以为是线程冲突的问题，开了一个新线程，但好像也不行。

正确的结果是触发按钮后，控制台会打印出从ini文件中读出来的内容才对，但现在没有，只有Print打印出来的三个S,能帮忙看看问题出在哪了，谢谢！