如何使用htaccess删除URL中的最后一个斜杠？

I'm trying to remove the last slash of my URLs.

I put this in my htaccess file :

RewriteRule ^(.+[^/])/$ http://%{HTTP_HOST}/$1 [R=301,L]

It works with files hosted in the root (or pages generated from the database), but it doesn't work with files hosted in subdirectories.

For example, I would like that this :

 http://mywebsite.com/dir1/dir2/dir3/index.php

goes to that :

 http://mywebsite.com/dir1/dir2/dir3

but it goes to that :

 http://mywebsite.com/[homeserver]/[server]/www/dir1/dir2/dir3

What is wrong with my htaccess file ?

Edit : here is the entire htaccess file

SetEnv PHP_VER 5
SetEnv REGISTER_GLOBALS 0
Options +FollowSymLinks

RewriteEngine on
RewriteBase /

RewriteRule ^(.+[^/])/$ http://%{HTTP_HOST}/$1 [R=301,L]

### Remove www
RewriteCond %{HTTP_HOST} !^mywebsite\.com$ [NC]
RewriteRule ^(.*)$ http://mywebsite.com/$1 [R=301,L]

### Get the URL in pathinfo mode
RewriteCond %{REQUEST_FILENAME} !-f 
RewriteCond %{REQUEST_FILENAME} !-d
RewriteRule (.*) /index.php/$1

### Redirect index.php to the root
RewriteRule ^index\.php$ http://mywebsite.com/ [R=301,L]

Try to add add below rule after RewriteEngine On and provide your full .htaccess for further analysis.

RewriteBase /

Have it like this:

SetEnv PHP_VER 5
SetEnv REGISTER_GLOBALS 0
Options +FollowSymLinks

RewriteEngine on
RewriteBase /

### Remove www
RewriteCond %{HTTP_HOST} ^www\.(.+)$ [NC]
RewriteRule ^ http://%1%{REQUEST_URI} [R=301,L,NE]

## Unless directory, remove trailing slash
RewriteCond %{REQUEST_FILENAME} !-d
RewriteRule ^(.+)/$ /$1 [NE,R=301,L]

### Redirect index.php from anywhere
# remove index.php
RewriteCond %{THE_REQUEST} /index\.php [NC]
RewriteCond %{REQUEST_URI} ^(.*/)index\.php$ [NC]
RewriteRule ^ %1 [L,R=301,NE]

### Get the URL in pathinfo mode
RewriteCond %{REQUEST_FILENAME} !-f 
RewriteCond %{REQUEST_FILENAME} !-d
RewriteRule .* index.php/$0 [L]

Make sure to clear your browser cache before testing this change.

You can use below code in .htacccess file to remove slash at the end of URL.

# remove trailing slash
RewriteCond %{REQUEST_FILENAME} !-d
RewriteCond %{THE_REQUEST} \s(.+?)/+[?\s]
RewriteRule ^(.+?)/$ /$1 [R=301,L]

I have used this and working fine.