运行命令如下：主要出现的错误是：
Error: Encountered internal Bowtie 2 exception (#1)
求助，搜索了很久不知道问题出在哪里。
bowtie2-build -f GCA_900519105.1_iwgsc_refseqv1.0_genomic.fna wheat
Settings:
Output files: "wheat.*.bt2l"
Line rate: 7 (line is 128 bytes)
Lines per side: 1 (side is 128 bytes)
Offset rate: 4 (one in 16)
FTable chars: 10
Strings: unpacked
Max bucket size: default
Max bucket size, sqrt multiplier: default
Max bucket size, len divisor: 4
Difference-cover sample period: 1024
Endianness: little
Actual local endianness: little
Sanity checking: disabled
Assertions: disabled
Random seed: 0
Sizeofs: void*:8, int:4, long:8, size_t:8
Input files DNA, FASTA:
GCA_900519105.1_iwgsc_refseqv1.0_genomic.fna
Building a LARGE index
Reading reference sizes
Time reading reference sizes: 00:02:46
Calculating joined length
Writing header
Reserving space for joined string
Could not allocate space for a joined string of 14271578887 elements.
Switching to a packed string representation.
Building a LARGE index
Reading reference sizes
Time reading reference sizes: 00:02:31
Calculating joined length
Writing header
Reserving space for joined string
Could not allocate space for a joined string of 14271578887 elements.
Please try running bowtie-build on a computer with more memory.
Total time for call to driver() for forward index: 00:05:17
Error: Encountered internal Bowtie 2 exception (#1)
Command: bowtie2-build --wrapper basic-0 -f GCA_900519105.1_iwgsc_refseqv1.0_genomic.fna wheat
Deleting "wheat.3.bt2l" file written during aborted indexing attempt.
Deleting "wheat.4.bt2l" file written during aborted indexing attempt.
Deleting "wheat.1.bt2l" file written during aborted indexing attempt.
Deleting "wheat.2.bt2l" file written during aborted indexing attempt.
Deleting "wheat.3.bt2l" file written during aborted indexing attempt.
Deleting "wheat.4.bt2l" file written during aborted indexing attempt.
Deleting "wheat.1.bt2l" file written during aborted indexing attempt.
Deleting "wheat.2.bt2l" file written during aborted indexing attempt.
是运行内存不够 小麦基因组太大了
博主的问题解决了吗？我也遇到了同样的问题